ycbF [2017-8-11 16:0:3]

glucarate dehydratase

locus: BSU02490

pI: 5.59

mw: 50.62 kDa

protein length: 455 aa

gene length: 1365 bp

function: glucarate utilization

product: glucarate dehydratase

essential: no

ec: 4.2.1.40

synonyms: gudD

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.14|Utilization of glucarate/galactarate]

Gene

Coordinates: 271,800 → 273,167

The protein

Catalyzed reaction/ biological activity

• d-glucarate = 5-dehydro-4-deoxy-D-glucarate + H₂O (according to Swiss-Prot)

Protein family

• GlucD subfamily (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|3542BC20C11985B3CFAA4F930E8D794700ADFD2E|ycbC]-[gene|5B66ADB81AB141982F187B1C5E3A9346AA064DE2|ycbD]-[gene|9C4932DA4DEF99263E57871A99684E3DAD93E1EC|ycbE]-[gene|372F2A420309DB0193AEF36A740EBA26410F97F2|ycbF]-[gene|1A65880F68898002EE8774F34EDC47F0243B7273|ycbG]-[gene|BA556E144975921DFDD598B0A7480C65176F0F14|ycbH]-[gene|349C99C258331962455100E2662DA2272E946C8A|ycbJ]
  description: [Pubmed|12044674]

  regulatory mechanism

  • [protein|1A65880F68898002EE8774F34EDC47F0243B7273|YcbG]: repression, [Pubmed|12044674], in [regulon|1A65880F68898002EE8774F34EDC47F0243B7273|YcbG regulon]
  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [Pubmed|18840696], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]

  regulation

  • induced by D-glucarate/galactarate ([protein|search|YcbG]) [Pubmed|12044674]

Biological materials

Mutant

• MGNA-C029 (ycbF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2027 NBRP B. subtilis, Japan]

References

• 12044674